Western blot
Sample: SH-SY5Y cells, 2.0x105 cells Primary dilution: 1:2500Secondary Antibody: HRP-conjugated goat anti-rabbit IgGSecondary dilution: 1:5000Image Submitted by: Atsuhiro TanabePharma Sci Kitasato UniversityWestern blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: OVCAR-3 cell lysate| Product Name | Rabbit anti-CASP7 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CMH-1, ICE-LAP3, MCH3 |
| Immunogen | The immunogen for anti-CASP7 antibody: synthetic peptide directed towards the N terminal of human CASP7 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogGoatHumanMousePigRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP7 |
|---|---|
| Gene Synonyms | CMH-1 ICE-LAP3 MCH3 |
| Gene Full Name | Caspase 7, apoptosis-related cysteine peptidase |
| Gene Summary | This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of this caspase is cleaved by caspase 3 and 10. It is activated upon cell death stimuli and induces apoptosis. Alternative splicing results in four transcript variants, encoding three distinct isoforms. |
| Alternative Names | CMH-1 ICE-LAP3 MCH3 |
| Molecular Weight(MW) | 37kDa |
| Sequence | 336 amino acids |
Western blot
Sample: SH-SY5Y cells, 2.0x105 cells Primary dilution: 1:2500Secondary Antibody: HRP-conjugated goat anti-rabbit IgGSecondary dilution: 1:5000Image Submitted by: Atsuhiro TanabePharma Sci Kitasato University
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: OVCAR-3 cell lysate| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM117867