Application
Western blot analysis of anti-MYLK3 Antibody (N-term) in A375 cell line lysates (35ug/lane). MYLK3 (arrow) was detected using the purified Pab.Application
Western blot analysis of anti-MYLK3 Antibody (N-term) in mouse heart tissue lysates (35ug/lane). MYLK3(arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MYLK3 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | MYLK3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MYLK3; MLCK; Putative myosin light chain kinase 3; Cardiac-MyBP-C-associated Ca/CaM kinase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MLCK |
|---|---|
| Alternative Names | MYLK3 MLCK Putative myosin light chain kinase 3 Cardiac-MyBP-C-associated Ca/CaM kinase |
| Molecular Weight(MW) | 88393 Da |
| Function | Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility (By similarity) |
| Tissue Specificity | Restricted to heart. |
| Cellular Localization | Cytoplasm (By similarity). |
Application
Western blot analysis of anti-MYLK3 Antibody (N-term) in A375 cell line lysates (35ug/lane). MYLK3 (arrow) was detected using the purified Pab.
Application
Western blot analysis of anti-MYLK3 Antibody (N-term) in mouse heart tissue lysates (35ug/lane). MYLK3(arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MYLK3 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
Data sheet for OM214882