Application
Confocal immunofluorescent analysis of Phospho-SMAD2-T220 Antibody(Cat#AP3675a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).Application
Dot blot analysis of anti-Phospho-SMAD2-T220 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Product Name | Phospho-SMAD2-T220 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; JV18-1; Mad-related protein 2; SMAD family member 2 |
| Modification | Phosphoralated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFDB |
| IF:1:10~50 DB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MADH2 MADR2 |
|---|---|
| Alternative Names | SMAD2 MADH2 MADR2 Mothers against decapentaplegic homolog 2 JV18-1 Mad-related protein 2 SMAD family member 2 |
| Molecular Weight(MW) | 52306 Da |
| Function | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator |
| Tissue Specificity | Expressed at high levels in skeletal muscle, heart and placenta |
| Cellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 |
| Entrez Gene | 4087 |
|---|
Application
Confocal immunofluorescent analysis of Phospho-SMAD2-T220 Antibody(Cat#AP3675a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Application
Dot blot analysis of anti-Phospho-SMAD2-T220 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.| Application Notes | IF:1:10~50 DB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
Data sheet for OM217732