Application
A. Western blot analysis on HeLa cell lysate using anti-Pol II RabMAb (Cat. #AJ1638a), dilution1:2000.| Product Name | Pol II Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | POLR2A, POLR2, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA-directed RNA polymerase II subunit RPB1 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR1509Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | POLR2 |
|---|---|
| Alternative Names | POLR2A POLR2 DNA-directed RNA polymerase II subunit RPB1 DNA-directed RNA polymerase II subunit A DNA-directed RNA polymerase III largest subunit RNA-directed RNA polymerase II subunit RPB1 |
| Molecular Weight(MW) | 217176 Da |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA- dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome |
| Tissue Specificity | A synthetic peptide corresponding to residues near the N-terminus of human Pol II was used as an immunogen. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 5430 |
|---|
Application
A. Western blot analysis on HeLa cell lysate using anti-Pol II RabMAb (Cat. #AJ1638a), dilution1:2000.| Application Notes | WB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Data sheet for OM218280