Application
A. Western blot analysis on Raji cell lysate using anti-B7-2 RabMAb (Cat. #AJ1072a), dilution 1:5000Application
B. Immunohistochemical staining of paraffin-embedded human tonsils using anti-B7-2 RabMAb (Cat. #AJ1072a).| Product Name | B7-2/CD86 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1158Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:10000~50000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD28LG2 |
|---|---|
| Alternative Names | CD86 CD28LG2 T-lymphocyte activation antigen CD86 Activation B7-2 antigen CTLA-4 counter-receptor B7.2 B70 FUN-1 BU63 |
| Molecular Weight(MW) | 37682 Da |
| Function | Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation |
| Tissue Specificity | Expressed by activated B-lymphocytes and monocytes |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 942 |
|---|
Application
A. Western blot analysis on Raji cell lysate using anti-B7-2 RabMAb (Cat. #AJ1072a), dilution 1:5000
Application
B. Immunohistochemical staining of paraffin-embedded human tonsils using anti-B7-2 RabMAb (Cat. #AJ1072a).| Application Notes | WB:1:10000~50000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Data sheet for OM227355