Application
Figure 1: Western blot analysis using anti-CD45 monoclonal antibody against truncated CD45 recombinant protein(1).Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lymph node tissue,showing membrane and cytoplasmic localization with DAB staining using anti-CD45 Antibody.| Product Name | CD45 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LCA; LY5; B220; CD45; T200; CD45R; GP180; PTPRC |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4A8A4C7A2 |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500 - 2000. IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD45 |
|---|---|
| Alternative Names | LCA LY5 B220 CD45 T200 CD45R GP180 PTPRC |
| Molecular Weight(MW) | 147254 Da |
| Function | Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN Dephosphorylates LYN, and thereby modulates LYN activity (By similarity) |
| Tissue Specificity | Purified recombinant fragment of CD45 expressed in E. Coli. |
| Cellular Localization | Membrane; Single-pass type I membrane protein. Membrane raft. Note=Colocalized with DPP4 in membrane rafts |
| Entrez Gene | 5788 |
|---|---|
| Nucleotide Accession | NP_563579.1 |
Application
Figure 1: Western blot analysis using anti-CD45 monoclonal antibody against truncated CD45 recombinant protein(1).
Application
Figure 2: Immunohistochemical analysis of paraffin-embedded human lymph node tissue,showing membrane and cytoplasmic localization with DAB staining using anti-CD45 Antibody.| Application Notes | WB:1:500 - 2000. IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of CD45 expressed in E. Coli. |
Data sheet for OM229238