Application
Western blot analysis of DCT Antibody (N-term) in K562(lane 1), A375(lane 2), Ramos(lane 3) cell line lysates (35ug/lane). DCT (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DCT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
DCT Antibody (N-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DCT Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DCT; TYRP2; L-dopachrome tautomerase; L-dopachrome Delta-isomerase; Tyrosinase-related protein 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TYRP2 |
|---|---|
| Alternative Names | DCT TYRP2 L-dopachrome tautomerase L-dopachrome Delta-isomerase Tyrosinase-related protein 2 |
| Molecular Weight(MW) | 59145 Da |
| Function | Involved in regulating eumelanin and phaeomelanin levels |
| Tissue Specificity | This DCT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68~98 amino acids from the N-term region of human DCT. |
| Cellular Localization | Melanosome membrane; Single-pass type I membrane protein |
| Entrez Gene | 1638 |
|---|
Application
Western blot analysis of DCT Antibody (N-term) in K562(lane 1), A375(lane 2), Ramos(lane 3) cell line lysates (35ug/lane). DCT (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DCT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
DCT Antibody (N-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Data sheet for OM231062