Application
Western blot analysis of APO-E in human brain tissue lysate with APO-E antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of APO E in mouse brain tissue with APO E Antibodyat 20 μg/mL.Application
Immunohistochemistry of APO E in mouse brain tissue with APO E Antibodyat 5 μg/mL.Application
Immunohistochemistry of APO-E inhuman liver tissue with APO-E antibody at 2.5 μg/ml.| Product Name | APO-E Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APO-E Antibody: AD2, LPG, LDLCQ5, Apolipoprotein E, Apo-E |
| Product description | APO-E Antibody: Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E (APO-E), a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. Defects in APO-E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.1) Vasquez EC, Peotta VA, Gava TMC, et al. Cardiac and vascular phenotypes in the epolipoprotein E-deficient mouse. J. Biomed. Sci. 2012; 19:22. |
| Immunogen | APO-E antibody was raised against a 19 amino acid peptide near the carboxy terminus of human APO-E. The immunogen is located within amino acids 220 - 270 of APO-E. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| APO-E antibody can be used for detection of APO-E by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APOE |
|---|---|
| Alternative Names | APO-E Antibody: AD2 LPG LDLCQ5 Apolipoprotein E Apo-E |
| Molecular Weight(MW) | Predicted: 35 kDa Observed: 34 kDa |
| Sequence Similarities | 2) Cat. No. 10-201 - Human Liver Tissue Slide |
Application
Western blot analysis of APO-E in human brain tissue lysate with APO-E antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of APO E in mouse brain tissue with APO E Antibodyat 20 μg/mL.
Application
Immunohistochemistry of APO E in mouse brain tissue with APO E Antibodyat 5 μg/mL.
Application
Immunohistochemistry of APO-E inhuman liver tissue with APO-E antibody at 2.5 μg/ml.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | APO-E antibody can be used for detection of APO-E by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | APO-E antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | APO-E Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM271325