Application
Western blot analysis of extract from rat brain, rat hippocampus and mouse brain using CD56 (NCAM).| Product Name | CD56 (NCAM) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD56, NCAM, MSK39, STK14A, NCAM1 |
| Product description | NCAM (neural cell adhesion molecule, CD56) is an adhesion glycoprotein with five extracellular immunoglobulin-like domains followed by two fibronectin type III repeats. Structural diversity is introduced by alternative splicing resulting in different cytoplasmic domains (1). NCAM mediates neuronal attachment, neurite extension and cell-cell interactions through homo and heterophilic interactions. PSA (polysialic acid) post-translationally modifies NCAM and increases the metastatic potential of small cell lung carcinoma, Wilms+ tumor, neuroblastoma and rhabdomyosarcoma (2). CD56 and CD16 are commonly used to identify NK cells although some cells with the T cell markers CD3 and CD4 also express CD56 (3).1) Cunningham, B. A. et al. (1987) Science 236, 799-806. Seidenfaden, R. et al. (2003) Mol. Cell. Biol. 23, 5908-5918. Robertson, M. J. and Ritz, J. (1990) Blood 76, 2421-2438. |
| Immunogen | CD56 (NCAM) antibody was raised against a peptide sequence around aa.850~854 (Q-T-K-E-N) derived from Human CD56 (NCAM). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NCAM1 |
|---|---|
| Alternative Names | CD56 NCAM MSK39 STK14A NCAM1 |
| Molecular Weight(MW) | 120 - 220 kDa |
| Tissue Specificity | This antibody detects endogenous levels of total NCAM protein. |
| Entrez Gene | 4684 |
|---|---|
| Protein Accession | NP_000606.3 |
Application
Western blot analysis of extract from rat brain, rat hippocampus and mouse brain using CD56 (NCAM).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM274780