Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.| Product Name | DIDO1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DIDO1, BYE1, DIO1, DATF1, DIDO2, DIDO3, DIO-1, DATF-1, C20orf158, dJ885L7.8 |
| Product description | In mice, the death inducer-obliterator-1 gene is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. DIDO1 gene is similar to the mouse gene and therefore is thought to be involved in apoptosis.Apoptosis, a major form of cell death, is an efficient mechanism for eliminating unwanted cells and is of central importance for development and homeostasis in metazoan animals. In mice, the death inducer-obliterator-1 gene is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. This gene is similar to the mouse gene and therefore is thought to be involved in apoptosis. Alternatively spliced transcripts have been found for this gene, encoding multiple isoforms.1) Futterer, A., et al., (2005) J. Clin. Invest. 115 (9), 2351-2362. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DIDO1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| DIDO1 antibody can be used for detection of DIDO1 by ELISA at 1:62500. DIDO1 antibody can be used for detection of DIDO1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DIDO1 |
|---|---|
| Alternative Names | DIDO1 BYE1 DIO1 DATF1 DIDO2 DIDO3 DIO-1 DATF-1 C20orf158 dJ885L7.8 |
| Molecular Weight(MW) | 59 kDa |
Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | DIDO1 antibody can be used for detection of DIDO1 by ELISA at 1:62500. DIDO1 antibody can be used for detection of DIDO1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store DIDO1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
Data sheet for OM277035