Application
Immunohistochemistry of ERp72 in rat colon tissue with ERp72 antibody at 5 μg/mL.Application
Immunofluorescence of ERp72 in rat colon tissue with ERp72 antibody at 20 μg/mL.Application
Western blot analysis of ERp72 in human colon tissue lysate with ERp72 antibody at (A) 1 and (B) 2 μg/ml.| Product Name | ERp72 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein disulfide isomerase family A member 4, Endoplasmic reticulum resident protein 70, Endoplasmic reticulum resident protein 72, ERp70, ERp-72 |
| Product description | Protein disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds (1,2). ER stress protein 72 (ERp72), also designated protein disulfide-isomerase A4, is involved in the catalysis of protein -S-S- bond rearrangement (3). It plays a role in the formation and isomerization of disulfide bonds (3,4).1) Huppa JB and Ploegh HL. The eS-Sence of -SH in the ER. Cell 1998; 92:145-8. |
| Immunogen | ERp72 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human ERp72. The immunogen is located within the last 50 amino acids of ERp72. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ERp72 antibody can be used for detection of ERp72 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDIA4 |
|---|---|
| Alternative Names | Protein disulfide isomerase family A member 4 Endoplasmic reticulum resident protein 70 Endoplasmic reticulum resident protein 72 ERp70 ERp-72 |
| Molecular Weight(MW) | Predicted: 71 kDa Observed: 75 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (80%) |
| Tissue Specificity | ERP72 antibody is human, mouse and rat reactive. |
Application
Immunohistochemistry of ERp72 in rat colon tissue with ERp72 antibody at 5 μg/mL.
Application
Immunofluorescence of ERp72 in rat colon tissue with ERp72 antibody at 20 μg/mL.
Application
Western blot analysis of ERp72 in human colon tissue lysate with ERp72 antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1320 - Human Colon Tissue Lysate |
|---|---|
| Application Notes | ERp72 antibody can be used for detection of ERp72 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ERp72 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | ERp72 antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM278191