Application
Flow cytometric analysis of HepG2 cells using FTH1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of FTH1 Antibody in A2058 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with FTH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | FTH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | FTH1 is the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases.1) Almeida,R.S., et.al., PLoS Pathog.(11), E1000217 (2008) |
| Immunogen | This FTH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the C-terminal region of human FTH1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACS,IHC-P,WB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FTH1 |
|---|---|
| Alternative Names | Ferritin heavy chain Ferritin H subunit Cell proliferation-inducing gene 15 protein Ferritin heavy chain N-terminally processed FTH1 FTH FTHL6 |
| Molecular Weight(MW) | 21 kDa |
Application
Flow cytometric analysis of HepG2 cells using FTH1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of FTH1 Antibody in A2058 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with FTH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM279250