Application
Immunohistochemistry of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 2.5 μg/ml.Application
Immunohistochemistry of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 1 μg/mL.Application
Western blot analysis of GFR alpha 1 in crude membrane fractions of human brain (B), liver (L), kidney (K), and spleen (S), respectively, with GFR alpha 1 antibody at 1 μg/mL.| Product Name | GFR alpha 1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | GFR alpha 1 Antibody: Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of kidneys and the enteric nerves system. Physiological responses to GDNF require the presence of a novel glycosylphosphadidylinositol linked protein GDNFRalpha, which is a cell surface receptor for GDNF. The cDNAs encoding GDNFRalpha from human, rat, chicken and mouse have been cloned recently. GDNFRalpha was also termed Ret ligand 1 (RETL1) or TGF-beta-related neurotrophic factor receptor 1 (TrnR1) and nominated as GFRα-1 recently. GFRα-1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK). Thus, GDNF, GFRα and the Ret PTK form a complex to transduce GDNF signal and to mediate GDNF function.1) Jing S, Wen D, Yu Y, et al. GDNF-induced activation of the Ret protein tyrosine kinase is mediated by GDNFR-a, a novel receptor for GDNF. Cell 1996; 85:1113-24. |
| Immunogen | GFR alpha 1 antibody was raised against a 14 amino acid peptide near the center of human GFR alpha 1. The immunogen is located within amino acids 360 - 410 of GFR alpha 1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| GFR alpha 1 antibody can be used for detection of GFR alpha 1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GFRA1 |
|---|---|
| Alternative Names | GFR alpha 1 Antibody: GDNFR RET1L RETL1 TRNR1 GDNFRA GFR-ALPHA-1 GDNF family receptor alpha-1 RET ligand 1 GDNF receptor alpha-1 |
| Molecular Weight(MW) | 51 kDa |
| Sequence Similarities | 4) Cat. No. 1306 - Human Spleen Tissue Lysate |
| Tissue Specificity | GFR alpha 1 antibody is predicted to not cross-react with other members of the GFR alpha family of proteins. |
Application
Immunohistochemistry of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 2.5 μg/ml.
Application
Immunohistochemistry of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 1 μg/mL.
Application
Western blot analysis of GFR alpha 1 in crude membrane fractions of human brain (B), liver (L), kidney (K), and spleen (S), respectively, with GFR alpha 1 antibody at 1 μg/mL.| Application Notes | GFR alpha 1 antibody can be used for detection of GFR alpha 1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. |
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| Form | Liquid |
|---|---|
| Storage Instructions | GFR alpha 1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | GFR alpha 1 Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM279708