Application
Immunohistochemistry of IL-36A in human brain tissue with IL-36A antibody at 5 μg/mL.Application
Immunofluorescence of IL-36A in human brain tissue with IL-36A antibody at 20 μg/mL.Application
Western blot analysis of IL-36A in MCF7 cell lysate with IL-36A antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | IL-36A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-36A Antibody: FIL1, FIL1E, IL1F6, IL-1F6, IL1(EPSILON), FIL1(EPSILON), IL1E, Interleukin-36 alpha, FIL1 epsilon, IL-1 epsilon |
| Product description | IL-36A is is a member of the interleukin 1 cytokine family whose gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2 (1). IL-36A is thought to activate the NF-kappaB pathway through IL-1 receptor family members IL-1RL2 and IL-1RAcP (2). Like the related proteins IL-36B and IL-36G, IL-36A requires post-translational processing for full agonist activity, but the cleavage mechanism is currently unknown (3). The IL-36 cytokines have been suggested to amplify Th1 responses by enhancing proliferation and Th1 polarization of naive CD4+ T cells (4).1) Smith DE, Renshaw BR, Ketchem RR, et al. Four new members expand the interleukin-1 superfamily. J. Biol. Chem. 2000; 275:1169-75. |
| Immunogen | IL-36A antibody was raised against an 11 amino acid peptide near the carboxy terminus of human IL-36A. The immunogen is located within the last 50 amino acids of IL-36A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| IL-36A antibody can be used for detection of IL-36A by Western blot at 1 - 2 μg/ml.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IL36A |
|---|---|
| Alternative Names | IL-36A Antibody: FIL1 FIL1E IL1F6 IL-1F6 IL1(EPSILON) FIL1(EPSILON) IL1E Interleukin-36 alpha FIL1 epsilon IL-1 epsilon |
| Molecular Weight(MW) | Predicted: 17 kDa Observed: 16 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | IL-36A antibody is human specific. IL-36A antibody will not cross-react with IL-36B or IL-36G. |
Application
Immunohistochemistry of IL-36A in human brain tissue with IL-36A antibody at 5 μg/mL.
Application
Immunofluorescence of IL-36A in human brain tissue with IL-36A antibody at 20 μg/mL.
Application
Western blot analysis of IL-36A in MCF7 cell lysate with IL-36A antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | IL-36A antibody can be used for detection of IL-36A by Western blot at 1 - 2 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | IL-36A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | IL-36A antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM281964