Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western Blot at 1:500 dilution + human fetal kidney lysate Lysates/proteins at 20 ug per lane.| Product Name | NEK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Serine/threonine-protein kinase Nek1, Never in mitosis A-related kinase 1, NimA-related protein kinase 1, Renal carcinoma antigen NY-REN-55, NEK1, KIAA1901 |
| Product description | Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.1) Surpili, M.J., et al., Biochemistry 42(51):15369-15376 (2003). |
| Immunogen | This NEK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1165-1196 amino acids from the C-terminal region of human NEK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:500 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | NEK1 |
|---|---|
| Alternative Names | Serine/threonine-protein kinase Nek1 Never in mitosis A-related kinase 1 NimA-related protein kinase 1 Renal carcinoma antigen NY-REN-55 NEK1 KIAA1901 |
| Molecular Weight(MW) | 143 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western Blot at 1:500 dilution + human fetal kidney lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:500 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM285962