Application
Western blot of rat cortex lysate showing specific immunolableing of the ~ 200k NF-H protein.| Product Name | Neurofilament NF-H Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFH, KIAA0845, NFH, 200 kDa neurofilament protein, NF-H |
| Product description | Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease.1) Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62. |
| Immunogen | Purified bovine NF-H. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | IHCWB |
| IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | ChickenMammalian species |
| Concentration | 1mg/ml |
| Gene Symbol | NEFH |
|---|---|
| Alternative Names | NFH KIAA0845 NFH 200 kDa neurofilament protein NF-H |
| Molecular Weight(MW) | 200 |
| Tissue Specificity | Specific for the ~200k Neurofilament H protein. It recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections. |
Application
Western blot of rat cortex lysate showing specific immunolableing of the ~ 200k NF-H protein.| Application Notes | IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Neurofilament NF-H antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM286037