| Product Name | NQO1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NAD(P)H Dehydrogenase (Quinine) 1 (NQO1); Quinone Reductase 1 (QR1); DT-Diaphorase (DTD); Azoreductase; Phylloquinone Reductase; Menadione Reductase; Dioxin-inducible 1; Disphorase-4 (DIA4); NAD(P)H:Quinone Oxireductase; NAD(P)H:Quinone Oxidoreductase 1 |
| Product description | NQO1 is a cytosolic antioxidant flavoprotein that catalyzes the reduction of highly reactive quinone metabolites and their derivatives by using NAD(P)H as an electron donor. Thus NQO1 acts as a detoxyfying enzyme and is involved in the body’s protection against oxidative stress. NQO1 acts as a protein chaperone, one of its targets being p53. In humans NQO1 is expressed at high levels in adipocytes and its expression levels are positively correlated with adiposity, glucose tolerance, and makers of liver dysfunction. Altered expression of NQO1 is associated with Alzheimer’s disease. NQO1 is abnormally elevated in many types of solid tumors and may represent a useful biomarker of pancreatic cancer. |
| Immunogen | Recombinant human NQO1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a,k |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: (direct and indirect: 1:2,000-1:5,000). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | NQO1 |
|---|---|
| Alternative Names | NAD(P)H Dehydrogenase (Quinine) 1 (NQO1) Quinone Reductase 1 (QR1) DT-Diaphorase (DTD) Azoreductase Phylloquinone Reductase Menadione Reductase Dioxin-inducible 1 Disphorase-4 (DIA4) NAD(P)H:Quinone Oxireductase NAD(P)H:Quinone Oxidoreductase 1 |
| Tissue Specificity | Recognizes human NQO1. Detects a band of ~32kDa by Western blot. |
| Application Notes | ELISA: (direct and indirect: 1:2,000-1:5,000). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for at least 1 year after receipt when stored at -20˚C. |
| Storage Buffer | Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives. |
Data sheet for OM286505