Application
Western blot analysis of P450R antibody in K562 cell line lysates (35ug/lane)Application
P450R Antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | P450R Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NADPH--cytochrome P450 reductase, CPR, P450R, POR, CYPOR |
| Product description | P450R is an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in its gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.1) Fukami,M., J. Clin. Endocrinol. Metab. 94 (5), 1723-1731 (2009) |
| Immunogen | This P450R antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 462-488 amino acids from the Central region of human P450R. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | POR |
|---|---|
| Alternative Names | NADPH--cytochrome P450 reductase CPR P450R POR CYPOR |
| Molecular Weight(MW) | 77 kDa |
Application
Western blot analysis of P450R antibody in K562 cell line lysates (35ug/lane)
Application
P450R Antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM287174