Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Product Name | PARP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Poly [ADP-ribose] polymerase 1, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1, PARP1, ADPRT, PPOL |
| Product description | This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.1) Majewski, P.M., et al. J. Biol. Chem. 285(45):34828-34838(2010) |
| Immunogen | This PARP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 590-618 amino acids from the Central region of human PARP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PARP1 |
|---|---|
| Alternative Names | Poly [ADP-ribose] polymerase 1 PARP-1 ADP-ribosyltransferase diphtheria toxin-like 1 ARTD1 NAD(+) ADP-ribosyltransferase 1 ADPRT 1 Poly[ADP-ribose] synthase 1 PARP1 ADPRT PPOL |
| Molecular Weight(MW) | 113 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM287381