Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Product Name | PDCD8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDCD8, AIF, CMT2D, CMTX4, COWCK, NADMR, NAMSD, PDCD8, COXPD6 |
| Product description | AIFM1 (PDCD8) is a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, AIFM1 induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9.This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Three alternative transcripts encoding different isoforms have been identified for this gene.1) Delettre, C., (er) J. Biol. Chem. (2006) In press. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDCD8. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PDCD8 antibody can be used for detection of PDCD8 by ELISA at 1:62500. PDCD8 antibody can be used for detection of PDCD8 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AIFM1 |
|---|---|
| Alternative Names | PDCD8 AIF CMT2D CMTX4 COWCK NADMR NAMSD PDCD8 COXPD6 |
| Molecular Weight(MW) | 36 kDa, 67 kDa, 66 kDa |
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | PDCD8 antibody can be used for detection of PDCD8 by ELISA at 1:62500. PDCD8 antibody can be used for detection of PDCD8 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PDCD8 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
Data sheet for OM287652