Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).Application
Confocal immunofluorescent analysis of UGT1A1 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).Application
UGT1A1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | UGT1A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | UDP-glucuronosyltransferase 1-1, UDPGT 1-1, UGT1*1, UGT1-01, UGT11, Bilirubin-specific UDPGT isozyme 1, hUG-BR1, UDP-glucuronosyltransferase 1-A, UGT-1A, UGT1A, UDP-glucuronosyltransferase 1A1, UGT1A1, GNT1, UGT1 |
| Product description | This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome.1) Italia, K.Y., et al. Clin. Biochem. 43 (16-17), 1329-1332 (2010) : |
| Immunogen | This UGT1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-90 amino acids from the N-terminal region of human UGT1A1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UGT1A1 |
|---|---|
| Alternative Names | UDP-glucuronosyltransferase 1-1 UDPGT 1-1 UGT1*1 UGT1-01 UGT11 Bilirubin-specific UDPGT isozyme 1 hUG-BR1 UDP-glucuronosyltransferase 1-A UGT-1A UGT1A UDP-glucuronosyltransferase 1A1 UGT1A1 GNT1 UGT1 |
| Molecular Weight(MW) | 60 kDa |
Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).
Application
Confocal immunofluorescent analysis of UGT1A1 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Application
UGT1A1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM294613