Application
Western blot analysis of extracts from 293 cells treated with etoposide using WEE1 (Phospho-Ser642) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | WEE1 (phospho Ser642) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | WEE1A, WEE1hu, kinase WEE1 |
| Product description | Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.1) Watanabe N., EMBO J. 14:1878-1891(1995). |
| Immunogen | WEE1 (phospho Ser642) antibody was raised against a peptide sequence around phosphorylation site of Serine 642 (S-V-S (p)-L-T) derived from Human WEE1. |
| Modification | Phosphoralated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WEE1 |
|---|---|
| Alternative Names | WEE1A WEE1hu kinase WEE1 |
| Molecular Weight(MW) | 100 kDa |
| Tissue Specificity | The antibody detects endogenous levels of WEE1 only when phosphorylated at serine 642. |
| Entrez Gene | 7465 |
|---|---|
| Protein Accession | NP_003381.1 |
Application
Western blot analysis of extracts from 293 cells treated with etoposide using WEE1 (Phospho-Ser642) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM295114