Application
Western blot analysis of ZIMP10 in K562 cell lysate with ZIMP10 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptideApplication
Immunofluorescence of ZIMP10 in K562 cells with ZIMP10 antibody at 20 μg/mL.Application
Immunocytochemistry of ZIMP10 in K562 cells with ZIMP10 antibody at 10 μg/mL.| Product Name | ZIMP10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ZIMP10 Antibody: ZIMP10, also known as ZMIZ1, is a novel PIAS (protein inhibitor of activated signal transducer and activator of transcription)-like protein initially identified as a transcriptional co-activator of the androgen receptor (AR). ZIMP10 and the related protein ZIMP7 interact with PIAS3 and enhances AR-mediated transcription. Later experiments showed that ZIMP10 is also a co-activator of the p53 tumor suppressor. Mice deficient in ZIMP10 result in embryonic lethality by E10.5; these embryos reveal severe defects in the reorganization of the yolk sac vascular plexus, indicating that ZIMP10 plays an important role in proper vascular development.1) Sharma M, Li X, Wang Y, et al. hZimp10 is an androgen receptor co-activator and forms a complex with sUMO-1 at replication foci. EMBO J. 2003; 22:6101-14. |
| Immunogen | ZIMP10 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ZIMP10. The immunogen is located within amino acids 90 - 140 of ZIMP10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| ZIMP10 antibody can be used for detection of ZIMP10 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ZMIZ1 |
|---|---|
| Alternative Names | ZIMP10 Antibody: MIZ RAI17 ZIMP10 hZIMP10 TRAFIP10 KIAA1224 Zinc finger MIZ domain-containing protein 1 PIAS-like protein Zimp10 |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
| Tissue Specificity | At least three isoforms are known to exist; this antibody will recognize all three. ZIMP10 antibody is predicted to not cross-react with other PIAS protein family members. |
Application
Western blot analysis of ZIMP10 in K562 cell lysate with ZIMP10 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide
Application
Immunofluorescence of ZIMP10 in K562 cells with ZIMP10 antibody at 20 μg/mL.
Application
Immunocytochemistry of ZIMP10 in K562 cells with ZIMP10 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | ZIMP10 antibody can be used for detection of ZIMP10 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | ZIMP10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ZIMP10 Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM295560