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Anti-PIK3CA antibody
Catalog Number:OM641771
OM641771
Discount Price
OM641771-100ul
1~2 Week
100ul
OM641771-50ul
50ul
OM641771-20ul
1~2 Week
20ul
1~2 Week
Product Profile
Product Name Anti-PIK3CA antibody
Antibody Type Primary Antibodies
Immunogen Polypeptide
Key Feature
Clonality polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications ELISAIHC
IHC:1:200-1:1000
Species Reactivity HumanMouseRat
Concentration 1mg/ml
Purification Protein A
Target Information
Gene Symbol PIK3CA
Gene Synonyms MCM
CWS5
MCAP
PI3K
CLAPO
CLOVE
MCMTC
PI3K-alpha
p110-alpha
Gene Full Name phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
Gene Summary Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22. [provided by RefSeq, Apr 2016]
Source Rabbit
Cellular Localization Cytosol,Plasma Membrane
Application

IHC

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PIK3CA antibody with DAB staining.Pre-treat the sections with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature for 30 minutes. Incubate the tissue with the primary antibody diluted at a ratio of 1:1500 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Use DAB(OM760029)as the chromogenic agent. Counterstain the tissue with hematoxylin, and mount the tissue sections with neutral gum.
Application Notes IHC:1:200-1:1000
Additional Information
Form Liquid
Storage Instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
Storage Buffer Purified antibody in PBS with 0.05% sodium azide.
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