WB
Western blot analysis of VEC using p-cPLA2 (S505) antibody. Antibody was diluted at 1:1000.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.ICC/IF
Immunofluorescence analysis of Hela cell. 1,cPLA2 (phospho Ser505) Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.| Product Name | cPLA2 (Phospho Ser505) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human c-PLA2 around the phosphorylation site of Ser505. AA range:471-520 |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLA2G4A |
|---|---|
| Gene Synonyms | GURDP cPLA2 PLA2G4 cPLA2-alpha |
| Gene Full Name | phospholipase A2 group IVA |
| Target Description | Phospho-cPLA2 (S505) Polyclonal Antibody detects endogenous levels of cPLA2 protein only when phosphorylated at S505.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):PLsPL |
| Molecular Weight(MW) | 110kDa |
| Cellular Localization | Cytoplasm,Golgi apparatus membrane,Nucleus envelope. |
WB
Western blot analysis of VEC using p-cPLA2 (S505) antibody. Antibody was diluted at 1:1000.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,cPLA2 (phospho Ser505) Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Data sheet for OM643846