WB
HEK-293 cells were subjected to SDS PAGE followed by western blot with RBM15 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.WB
SW480 cells were subjected to SDS PAGE followed by western blot with RBM15 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using RBM15 antibody at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBM15 antibody at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L), CL594-phalloidin (red).IP
IP result of anti-RBM15 (IP:4ug; Detection:1:500) with HEK-293 cells lysate 2600 ug.| Product Name | RBM15 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human RBM15 protein |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:2000-1:16000 IHC:1:50-1:500 ICC/IF:1:1000-1:4000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RBM15 |
|---|---|
| Gene Synonyms | OTT OTT1 SPEN |
| Gene Full Name | RNA binding motif protein 15 |
| Gene Summary | Members of the SPEN (Split-end) family of proteins, including RBM15, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components (Hiriart et al., 2005 [PubMed 16129689]).[supplied by OMIM, Feb 2009] |
| Molecular Weight(MW) | 107kDa |
| Cellular Localization | Nucleus. |
WB
HEK-293 cells were subjected to SDS PAGE followed by western blot with RBM15 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB
SW480 cells were subjected to SDS PAGE followed by western blot with RBM15 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using RBM15 antibody at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBM15 antibody at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L), CL594-phalloidin (red).
IP
IP result of anti-RBM15 (IP:4ug; Detection:1:500) with HEK-293 cells lysate 2600 ug.| Application Notes | WB:1:2000-1:16000 IHC:1:50-1:500 ICC/IF:1:1000-1:4000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Data sheet for OM643876