WB
Western Blot analysis of KB cells using Acetyl-Histone H4 (K16) Polyclonal Antibody. Secondary antibody was diluted at 1:20000.IHC
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.ICC/IF
Immunofluorescence analysis of Hela cell. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). α-tubulin Monoclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).| Product Name | Histone H4 (Acetyl Lys16) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Histone H4 around the acetylated site of Lys16. AA range:1-50. |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:300 ICC/IF:1:50-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H4C1-16 |
|---|---|
| Gene Synonyms | H4c1 H4c2 H4c3 H4c4 H4c6 H4c8 H4c9 H4c11 H4c12 H4c14 H4c16 |
| Gene Full Name | H4 clustered histone |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. [provided by RefSeq, Sep 2015] |
| Target Description | Acetyl-Histone H4 (K16) Polyclonal Antibody detects endogenous levels of Histone H4 protein only when acetylated at K16.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites) |
| Molecular Weight(MW) | 11kDa |
| Cellular Localization | Nucleus. Chromosome. |
WB
Western Blot analysis of KB cells using Acetyl-Histone H4 (K16) Polyclonal Antibody. Secondary antibody was diluted at 1:20000.
IHC
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,Histone H4 (Acetyl Lys16) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). α-tubulin Monoclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:300 ICC/IF:1:50-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
Data sheet for OM643921