WB
Sample: Gradient diluted antigen protein Electrophoresis: 10% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, voltage 10v overnight Closed: 5% skimmed milk powder, incubated at room temperature for 1 hour Primary antibody: diluted 1:5000, incubated at room temperature for 1 hour| Product Name | Anti-RPOB/ DNA-directed RNA polymerase subunit beta |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis ATCG00190，P50546 |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Purification | Affinity purified |
| Gene Symbol | rpoB |
|---|---|
| Gene Full Name | DNA-directed RNA polymerase subunit beta |
| Target Description | RPOB encodes chloroplast DNA-dependent RNA polymerase B subunit. It catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. |
| Molecular Weight(MW) | 121kDa for Arabidopsis |
WB
Sample: Gradient diluted antigen protein Electrophoresis: 10% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, voltage 10v overnight Closed: 5% skimmed milk powder, incubated at room temperature for 1 hour Primary antibody: diluted 1:5000, incubated at room temperature for 1 hour| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
Data sheet for OM643930