WB
Western blot analysis using ATP5F1A mouse mAb against HepG2 (1), Hela (2), HCT116 (3), mouse heart (4), and rat heart (5) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using ATP5F1A mouse mAb with DAB staining.FC
Flow cytometric analysis of Jurkat cells using ATP5F1A mouse mAb (green) and negative control (red).| Product Name | Mouse Monoclonal Antibody to ATP5F1A |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human ATP5F1A (AA: 44-220) expressed in E. Coli. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCIHCWB |
| WB:1:500-1:2000 IHC:1:200-1:1000 FC:1:200-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP5F1A |
|---|---|
| Gene Synonyms | OMR ORM ATPM MOM2 ATP5A hATP1 ATP5A1 MC5DN4 ATP5AL2 COXPD22 MC5DN4A MC5DN4B HEL-S-123m |
| Gene Full Name | ATP synthase F1 subunit alpha |
| Gene Summary | This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq, Mar 2012] |
| Molecular Weight(MW) | 59kDa |
| Cellular Localization | Cell membrane,CF(1),Membrane,Mitochondrion,Mitochondrion inner membrane. |
WB
Western blot analysis using ATP5F1A mouse mAb against HepG2 (1), Hela (2), HCT116 (3), mouse heart (4), and rat heart (5) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using ATP5F1A mouse mAb with DAB staining.
FC
Flow cytometric analysis of Jurkat cells using ATP5F1A mouse mAb (green) and negative control (red).| Application Notes | WB:1:500-1:2000 IHC:1:200-1:1000 FC:1:200-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
Data sheet for OM643970