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Mouse Monoclonal Antibody to ATP5F1A
Catalog Number:OM643970
OM643970
Discount Price
OM643970-100ul
100ul
OM643970-50ul
50ul
Product Profile
Product Name Mouse Monoclonal Antibody to ATP5F1A
Antibody Type Primary Antibodies
Immunogen Purified recombinant fragment of human ATP5F1A (AA: 44-220) expressed in E. Coli.
Key Feature
Clonality monoclonal
Isotype IgG1
Host Species Mouse
Tested Applications FCIHCWB
WB:1:500-1:2000
IHC:1:200-1:1000
FC:1:200-1:400
Species Reactivity HumanMouseRat
Concentration 1mg/ml
Purification Affinity purified
Target Information
Gene Symbol ATP5F1A
Gene Synonyms OMR
ORM
ATPM
MOM2
ATP5A
hATP1
ATP5A1
MC5DN4
ATP5AL2
COXPD22
MC5DN4A
MC5DN4B
HEL-S-123m
Gene Full Name ATP synthase F1 subunit alpha
Gene Summary This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq, Mar 2012]
Molecular Weight(MW) 59kDa
Cellular Localization Cell membrane,CF(1),Membrane,Mitochondrion,Mitochondrion inner membrane.
Application

WB

Western blot analysis using ATP5F1A mouse mAb against HepG2 (1), Hela (2), HCT116 (3), mouse heart (4), and rat heart (5) cell lysate.

IHC

Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using ATP5F1A mouse mAb with DAB staining.

FC

Flow cytometric analysis of Jurkat cells using ATP5F1A mouse mAb (green) and negative control (red).
Application Notes WB:1:500-1:2000
IHC:1:200-1:1000
FC:1:200-1:400
Additional Information
Form Liquid
Storage Instructions Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Storage Buffer Purified antibody in PBS with 0.05% sodium azide.
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