WB
Positive WB detected in: Mouse liver tissue All, lanes: GJB2 antibody at 3µg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.IHC
Immunohistochemistry of paraffin-embedded human gastric cancer using GJB2 antibody at dilution of 1:100.ICC/IF
Immunofluorescent analysis of MCF-7 cells using GJB2 antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-Homo sapiens (Human) GJB2 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Gap junction beta-2 protein (99-131AA) |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | GJB2 |
|---|---|
| Gene Synonyms | HID KID PPK BAPS CX26 DFNA3 DFNB1 NSRD1 DFNA3A DFNB1A |
| Gene Full Name | gap junction protein beta 2 |
| Gene Summary | This gene encodes a member of the gap junction protein family. The gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels that facilitate the transfer of ions and small molecules between cells. The gap junction proteins, also known as connexins, purified from fractions of enriched gap junctions from different tissues differ. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene are responsible for as much as 50% of pre-lingual, recessive deafness. [provided by RefSeq, Oct 2008] |
| Molecular Weight(MW) | 27kDa |
| Cellular Localization | Cell membrane. |
WB
Positive WB detected in: Mouse liver tissue All, lanes: GJB2 antibody at 3µg/ml, Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.
IHC
Immunohistochemistry of paraffin-embedded human gastric cancer using GJB2 antibody at dilution of 1:100.
ICC/IF
Immunofluorescent analysis of MCF-7 cells using GJB2 antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:1000-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300,Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Data sheet for OM644256