WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PPARα antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2, Lane 2: MCF7, Lane 3: NIH-3T3, Lane 4: L6.IHC
Human liver was stained with anti-PPARα rabbit antibody.IHC
Rat liver was stained with anti-PPARα rabbit antibody.ICC/IF
Immunofluorescence analysis of Hela cell. 1,PPAR-α Antibody(red) was diluted at 1:200(4° overnight). Galectin-3 Monoclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).| Product Name | PPARα Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:5000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PPARA |
|---|---|
| Gene Synonyms | PPAR NR1C1 hPPAR PPARalpha PPAR-alpha |
| Gene Full Name | peroxisome proliferator activated receptor alpha |
| Gene Summary | Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 52kDa |
| Cellular Localization | Nucleus. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PPARα antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: HepG2, Lane 2: MCF7, Lane 3: NIH-3T3, Lane 4: L6.
IHC
Human liver was stained with anti-PPARα rabbit antibody.
IHC
Rat liver was stained with anti-PPARα rabbit antibody.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,PPAR-α Antibody(red) was diluted at 1:200(4° overnight). Galectin-3 Monoclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).| Application Notes | WB:1:1000-1:5000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
Data sheet for OM644264