WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-RUNX2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: MDA-MB-231, Lane 2: Saos-2, Lane 3: NIH-3T3, Lane 4: Rat womb.IHC
Human osteosarcoma was stained with anti-RUNX2 rabbit antibody.IHC
Mouse spleen was stained with anti-RUNX2 rabbit antibody.ICC/IF
Immunofluorescence analysis of Hela cell. 1,RUNX2 Antibody(red) was diluted at 1:200(4° overnight). NSE Monoclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).| Product Name | RUNX2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:10000 IHC:1:1000-1:5000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RUNX2 |
|---|---|
| Gene Synonyms | CCD AML3 CCD1 CLCD OSF2 CBFA1 OSF-2 PEA2aA PEBP2aA CBF-alpha-1 |
| Gene Full Name | RUNX family transcription factor 2 |
| Gene Summary | This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2016] |
| Molecular Weight(MW) | 57kDa |
| Cellular Localization | Nucleus. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-RUNX2 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: MDA-MB-231, Lane 2: Saos-2, Lane 3: NIH-3T3, Lane 4: Rat womb.
IHC
Human osteosarcoma was stained with anti-RUNX2 rabbit antibody.
IHC
Mouse spleen was stained with anti-RUNX2 rabbit antibody.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,RUNX2 Antibody(red) was diluted at 1:200(4° overnight). NSE Monoclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).| Application Notes | WB:1:2000-1:10000 IHC:1:1000-1:5000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
Data sheet for OM644266