All products Antibodies Alpaca anti-rabbit IgG-HRP

Alpaca anti-rabbit IgG-HRP

Catalog Number:OM626369

  • Western blot analysis of rabbit anti-GAPDH antibody (ET1601-4,1/2000) and rabbit anti-GST3 antibody (ER1802-63, 1/1000) with K562 Cell and A549 Cell lysates (All lanes proteins at 10

  • Immunohistochemical analysis of paraffin-embedded human Kidney tissue using rabbit anti-CD13 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-45, 1/200) for 30 minutes at room temperature. Alpaca anti-rabbit IgG FC, recombinant VHH (HRP) (HA1031, 1/200 dilution) was used for 30 min at room temperature. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Profile

Product Name Alpaca anti-rabbit IgG-HRP
Antibody Type Secondary Antibodies
Product description
Higher resolution imaging due to the Alpaca anti-rabbit IgG FC, recombinant VHHs’ 10 times smaller size than conventional secondary antibodies. The small size enables better tissue penetration, antigen access and decreases the distance between the epitope and label.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Camel
Tested Applications

ELISAIHCIPWB

IP: Use at an assay dependent concentration. ELISA: 1/5,000-1/20,000 IHC: 1/100-1/500 WB: 1/10,000-1/100,000:
Species Reactivity

Rabbit

Concentration 1mg/ml
Purification Affinity purified

Application

  • Western blot

    Western blot analysis of rabbit anti-GAPDH antibody (ET1601-4,1/2000) and rabbit anti-GST3 antibody (ER1802-63, 1/1000) with K562 Cell and A549 Cell lysates (All lanes proteins at 10

  • IHC

    Immunohistochemical analysis of paraffin-embedded human Kidney tissue using rabbit anti-CD13 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-45, 1/200) for 30 minutes at room temperature. Alpaca anti-rabbit IgG FC, recombinant VHH (HRP) (HA1031, 1/200 dilution) was used for 30 min at room temperature. Tissues were counterstained with hematoxylin and mounted with DPX.

Application Notes
IP: Use at an assay dependent concentration. ELISA: 1/5,000-1/20,000 IHC: 1/100-1/500 WB: 1/10,000-1/100,000:

Additional Information

Form Liquid
Storage Instructions Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer 1*PBS (pH7.4), 0.5%BSA, 50%Glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

  • Catalog Number: OM626369
  • Price: $180.00
  • Discount Price
  • Size:
  • Store: In stock
  • Lead time: 2~3 Week
  • Quantity:
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