Western blot analysis of rabbit anti-GAPDH antibody (ET1601-4,1/2000) and rabbit anti-GST3 antibody (ER1802-63, 1/1000) with K562 Cell and A549 Cell lysates (All lanes proteins at 10
Immunohistochemical analysis of paraffin-embedded human Kidney tissue using rabbit anti-CD13 antibody.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-45, 1/200) for 30 minutes at room temperature. Alpaca anti-rabbit IgG FC, recombinant VHH (HRP) (HA1031, 1/200 dilution) was used for 30 min at room temperature. Tissues were counterstained with hematoxylin and mounted with DPX.
Higher resolution imaging due to the Alpaca anti-rabbit IgG FC, recombinant VHHs’ 10 times smaller size than conventional secondary antibodies. The small size enables better tissue penetration, antigen access and decreases the distance between the epitope and label.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Camel
Tested Applications
ELISAIHCIPWB
IP: Use at an assay dependent concentration.
ELISA: 1/5,000-1/20,000
IHC: 1/100-1/500
WB: 1/10,000-1/100,000:
Species Reactivity
Rabbit
Concentration
1mg/ml
Purification
Affinity purified
Application
Western blot
Western blot analysis of rabbit anti-GAPDH antibody (ET1601-4,1/2000) and rabbit anti-GST3 antibody (ER1802-63, 1/1000) with K562 Cell and A549 Cell lysates (All lanes proteins at 10
IHC
Immunohistochemical analysis of paraffin-embedded human Kidney tissue using rabbit anti-CD13 antibody.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1803-45, 1/200) for 30 minutes at room temperature. Alpaca anti-rabbit IgG FC, recombinant VHH (HRP) (HA1031, 1/200 dilution) was used for 30 min at room temperature. Tissues were counterstained with hematoxylin and mounted with DPX.
Application Notes
IP: Use at an assay dependent concentration.
ELISA: 1/5,000-1/20,000
IHC: 1/100-1/500
WB: 1/10,000-1/100,000:
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.5%BSA, 50%Glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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