All products Antibodies Anti-biotin (D5A7) Rabbit mAb (HRP Conjugate)

Anti-biotin (D5A7) Rabbit mAb (HRP Conjugate)

Catalog Number：OM296294

  • Western blot analysis of extracts from HeLa cells, untreated or treated with Staurosporine (1 μM, 3 hr) using Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb (Biotinylated) primary antibody and Anti-Biotin (D5A7) Rabbit mAb (HRP Conjugate) as secondary detection antibody.

Product Profile

Product Name Anti-biotin (D5A7) Rabbit mAb (HRP Conjugate)
Antibody Type Secondary Antibodies
Product description 
Anti-biotin (D5A7) Rabbit mAb is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. This product has been optimized to detect biotinylated primary antibodies. HRP conjugated antibodies do not require incubation with a secondary antibody. *Do not mix this antibody in solution with any Anti-rabbit antibody. Anti-rabbit antibodies will cross react with this antibody and could result in decreased activity of both Anti-rabbit and Anti-biotin (D5A7) Rabbit mAb.

Key Feature

Clonality Monoclonal
Tested Applications

WBIPIHCIFFCChIP
Species Reactivity

Biotin
Concentration 1 mg/ml
Purification

Target Information

Tissue Specificity

Anti-biotin (D5A7) Rabbit mAb (HRP Conjugate) recognizes biotin attached to proteins, peptides, oligonucleotides and solid matrices.

Species Reactivity: All Species Expected

Application

  • Application

    Western blot analysis of extracts from HeLa cells, untreated or treated with Staurosporine (1 μM, 3 hr) using Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb (Biotinylated) primary antibody and Anti-Biotin (D5A7) Rabbit mAb (HRP Conjugate) as secondary detection antibody.

Additional Information

Storage Instructions
Application Dilutions
Western Blotting 1:1000
Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml bovine serum albumin (BSA), and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM296294
  • Price: $150.00
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  • Lead time: 4~6 Week

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