Confocal immunofluorescent analysis of mouse embryonic stem cells growing on mouse embryonic fibroblast (MEF) feeder cells using SSEA1 (MC480) Mouse mAb detected with anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).
Flow cytometric analysis of untreated Jurkat cells using Akt (5G3) Mouse mAb detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) (green) compared to a nonspecific negative control antibody (red).
Anti-Mouse IgG (H+L) F(ab')2 Fragment antibody was conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.
Key Feature
Clonality
Monoclonal
Isotype
Cocktail
Host Species
Alligator
Tested Applications
ChIPFCIFIHCIPWB
WB:1:1000~1:5000
Species Reactivity
Mouse
Concentration
0.2 mg/ml
Purification
Affinity purified
Target Information
Tissue Specificity
F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against human IgG and human serum.
Application
Application
Confocal immunofluorescent analysis of mouse embryonic stem cells growing on mouse embryonic fibroblast (MEF) feeder cells using SSEA1 (MC480) Mouse mAb detected with anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).
Application
Flow cytometric analysis of untreated Jurkat cells using Akt (5G3) Mouse mAb detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) (green) compared to a nonspecific negative control antibody (red).
Application Notes
WB:1:1000~1:5000
Additional Information
Form
Liquid
Storage Instructions
The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays.
Storage:
Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
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