All products Antibodies Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate)

Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate)

Catalog Number:OM296278

  • Confocal immunofluorescent analysis of mouse embryonic stem cells growing on mouse embryonic fibroblast (MEF) feeder cells using SSEA1 (MC480) Mouse mAb detected with anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).

  • Flow cytometric analysis of untreated Jurkat cells using Akt (5G3) Mouse mAb detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) (green) compared to a nonspecific negative control antibody (red).

Product Profile

Product Name Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate)
Antibody Type Secondary Antibodies
Product description
Anti-Mouse IgG (H+L) F(ab')2 Fragment antibody was conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.

Key Feature

Clonality Monoclonal
Isotype Cocktail
Host Species Alligator
Tested Applications

ChIPFCIFIHCIPWB

WB:1:1000~1:5000
Species Reactivity

Mouse

Concentration 0.2 mg/ml
Purification Affinity purified

Target Information

Tissue Specificity F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against human IgG and human serum.

Application

  • Application

    Confocal immunofluorescent analysis of mouse embryonic stem cells growing on mouse embryonic fibroblast (MEF) feeder cells using SSEA1 (MC480) Mouse mAb detected with anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).

  • Application

    Flow cytometric analysis of untreated Jurkat cells using Akt (5G3) Mouse mAb detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) (green) compared to a nonspecific negative control antibody (red).

Application Notes
WB:1:1000~1:5000

Additional Information

Form Liquid
Storage Instructions The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays. Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

  • Catalog Number: OM296278
  • Price: $150.00
  • Discount Price
  • Size:
  • Store: Out of stock
  • Lead time: 4~6 Week
  • Quantity:
    - +
  • Shipping to (Please select)
Search the catalog number from any major antibody supplier/brand in the search bar above. The search engine will return OmnimAbs products for these targets.

2013 © Omnimabs , All Rights Reserved.