Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin Mouse mAb detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) (blue). Red = propidium iodide (fluorescent DNA dye).
Flow cytometric analysis of untreated Jurkat cells using Akt Mouse mAb detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) (green) compared to a nonspecific negative control antibody (red).
Anti-Mouse IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions and formulated at 1 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.
Key Feature
Clonality
Monoclonal
Isotype
Cocktail
Host Species
Goat
Tested Applications
ChIPFCIFIHCIPWB
The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays.:
Species Reactivity
Mouse
Concentration
1 mg/ml
Purification
Affinity purified
Target Information
Tissue Specificity
F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against human IgG and human serum.
Application
Application
Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin Mouse mAb detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) (blue). Red = propidium iodide (fluorescent DNA dye).
Application
Flow cytometric analysis of untreated Jurkat cells using Akt Mouse mAb detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) (green) compared to a nonspecific negative control antibody (red).
Application Notes
The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays.:
Additional Information
Storage Instructions
Storage:
Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
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