All products Antibodies Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate)

Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate)

Catalog Number:OM296276

  • Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin Mouse mAb detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) (blue). Red = propidium iodide (fluorescent DNA dye).

  • Flow cytometric analysis of untreated Jurkat cells using Akt Mouse mAb detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) (green) compared to a nonspecific negative control antibody (red).

Product Profile

Product Name Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate)
Antibody Type Secondary Antibodies
Product description
Anti-Mouse IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions and formulated at 1 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.

Key Feature

Clonality Monoclonal
Isotype Cocktail
Host Species Goat
Tested Applications

ChIPFCIFIHCIPWB

The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays.:
Species Reactivity

Mouse

Concentration 1 mg/ml
Purification Affinity purified

Target Information

Tissue Specificity F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against human IgG and human serum.

Application

  • Application

    Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin Mouse mAb detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) (blue). Red = propidium iodide (fluorescent DNA dye).

  • Application

    Flow cytometric analysis of untreated Jurkat cells using Akt Mouse mAb detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) (green) compared to a nonspecific negative control antibody (red).

Application Notes
The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays.:

Additional Information

Storage Instructions Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

  • Catalog Number: OM296276
  • Price: $150.00
  • Discount Price
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  • Store: Out of stock
  • Lead time: 4~6 Week
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