Anti-mouse IgG (H+L), F(ab') 2 Fragment (DyLight ® 488 Conjugate)
Antibody Type
Secondary Antibodies
Product description
This Cell Signaling Technology antibody was conjugated to DyLight® 488 fluorescent dye under optimal conditions and tested in-house for indirect flow cytometric and immunofluorescent analysis of human and mouse cells.
Key Feature
Clonality
Monoclonal
Isotype
Cocktail
Host Species
Alligator
Tested Applications
FCIF
For flow cytometry, use product at a 1:1000 dilution. For immunofluorescence, the optimal dilution may have to be determined. For the detection of many antibodies, the optimal dilution is 1:250-1:1000.:
Species Reactivity
Mouse
Concentration
1 mg/ml
Purification
Affinity purified
Target Information
Tissue Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, mouse IgG and mouse serum. No reaction was observed against anti-pepsin, anti-goat IgG F(c) or human serum proteins.
Application
Application Notes
For flow cytometry, use product at a 1:1000 dilution. For immunofluorescence, the optimal dilution may have to be determined. For the detection of many antibodies, the optimal dilution is 1:250-1:1000.:
Additional Information
Form
Liquid
Storage Instructions
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mg/ml BSA. Store at 4°C. Protect from light. Do not freeze.
Storage Buffer
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mg/ml BSA. Store at 4°C. Protect from light. Do not freeze.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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