All products Antibodies Anti-mouse IgG (H+L), F(ab') 2 Fragment (Sepharose ® Bead Conjugate)

Anti-mouse IgG (H+L), F(ab') 2 Fragment (Sepharose ® Bead Conjugate)

Catalog Number:OM296275

  • Immunoprecipitation of COS-7 cell extracts, untransfected or transfected with a construct overexpressing TrCP1-GST using GST (26H1) Mouse mAb #2624. Extracts were incubated with either Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Sepharose ® Bead Conjugate) (lane 1 and 2) or Protein G Beads (lane 3). The western blot was probed using GST (91G1) Rabbit mAb #2625.

  • Immunoprecipitation of NIH/3T3 cell extracts using Akt (pan)(40D4) Mouse mAb #2920. Extracts were incubated with either Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Sepharose ® Bead Conjugate) (lane 1) or Protein G Beads (lane 2). The western blot was probed using Akt (pan) (C67E7) Rabbit mAb #4691.

Product Profile

Product Name Anti-mouse IgG (H+L), F(ab') 2 Fragment (Sepharose ® Bead Conjugate)
Antibody Type Secondary Antibodies
Product description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Anti-mouse IgG (H+L), F(ab')2 Fragment (Sepharose Bead Conjugate) is useful for the immunoprecipitation of antibodies raised in mice.

Key Feature

Clonality Monoclonal
Tested Applications

WBIPIHCIFFCChIP

Species Reactivity

Mouse

Concentration 1 mg/ml
Purification

Target Information

Tissue Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, mouse IgG and mouse serum. No reaction was observed against anti-pepsin, anti-goat IgG Fc, or human serum proteins.

Application

  • Application

    Immunoprecipitation of COS-7 cell extracts, untransfected or transfected with a construct overexpressing TrCP1-GST using GST (26H1) Mouse mAb #2624. Extracts were incubated with either Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Sepharose ® Bead Conjugate) (lane 1 and 2) or Protein G Beads (lane 3). The western blot was probed using GST (91G1) Rabbit mAb #2625.

  • Application

    Immunoprecipitation of NIH/3T3 cell extracts using Akt (pan)(40D4) Mouse mAb #2920. Extracts were incubated with either Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Sepharose ® Bead Conjugate) (lane 1) or Protein G Beads (lane 2). The western blot was probed using Akt (pan) (C67E7) Rabbit mAb #4691.

Additional Information

Storage Instructions

Add 10-20 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

  • Catalog Number: OM296275
  • Price: $150.00
  • Discount Price
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  • Store: Out of stock
  • Lead time: 4~6 Week
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