Immunoprecipitation of COS-7 cell extracts, untransfected or transfected with a construct overexpressing TrCP1-GST using GST (26H1) Mouse mAb #2624. Extracts were incubated with either Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Sepharose ® Bead Conjugate) (lane 1 and 2) or Protein G Beads (lane 3). The western blot was probed using GST (91G1) Rabbit mAb #2625.
Immunoprecipitation of NIH/3T3 cell extracts using Akt (pan)(40D4) Mouse mAb #2920. Extracts were incubated with either Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Sepharose ® Bead Conjugate) (lane 1) or Protein G Beads (lane 2). The western blot was probed using Akt (pan) (C67E7) Rabbit mAb #4691.
Product Name | Anti-mouse IgG (H+L), F(ab') 2 Fragment (Sepharose ® Bead Conjugate) |
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Antibody Type | Secondary Antibodies |
Product description |
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Clonality | Monoclonal |
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Tested Applications | |
Species Reactivity | |
Concentration | 1 mg/ml |
Purification |
Tissue Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, mouse IgG and mouse serum. No reaction was observed against anti-pepsin, anti-goat IgG Fc, or human serum proteins. |
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Immunoprecipitation of COS-7 cell extracts, untransfected or transfected with a construct overexpressing TrCP1-GST using GST (26H1) Mouse mAb #2624. Extracts were incubated with either Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Sepharose ® Bead Conjugate) (lane 1 and 2) or Protein G Beads (lane 3). The western blot was probed using GST (91G1) Rabbit mAb #2625.
Immunoprecipitation of NIH/3T3 cell extracts using Akt (pan)(40D4) Mouse mAb #2920. Extracts were incubated with either Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Sepharose ® Bead Conjugate) (lane 1) or Protein G Beads (lane 2). The western blot was probed using Akt (pan) (C67E7) Rabbit mAb #4691.
Storage Instructions | Add 10-20 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details. Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies. |
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Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
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