All products Antibodies Anti-rabbit IgG (H+L) (DyLight™ 680 Conjugate)

Anti-rabbit IgG (H+L) (DyLight™ 680 Conjugate)

Catalog Number:OM296285

  • Western blot analysis of Jurkat cell lysates (#9194) treated with either U0126 (MEK 1/2 inhibitor) or TPA (12-O-Tetradecanoylphorbol-13-Acetate) using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb detected with Anti-rabbit IgG (H+L) (DyLight™ 680 Conjugate) (red) and Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb detected with Anti-mouse IgG (H+L) (DyLight™ 800 Conjugate) (green). The array image pixel intensities obtained using a LI-COR ® Biosciences Odyssey ® Infrared Imaging System are shown in the upper panel while corresponding fluorescent western blots are shown in the lower panel.

  • In-Cell Western™ analysis of A549 cells exposed to varying concentrations of U0126 (MEK1/2 Inhibitor) for 3 hours, followed by TPA (Phorbol-12-Myristate-13-Acetate) stimulation for 30 minutes. With increasing concentrations of U0126, a significant decrease (~5 fold) in Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb signal as compared to the TPA-stimulated control was observed. When using phospho-Erk as a measurement, the IC 50 of this compound was 1.9 μM. Data and images were generated on the LI-COR ® Biosciences Odyssey ® Infrared Imaging System using Anti-rabbit IgG (H+L) (DyLight™ 680 Conjugate).

Product Profile

Product Name Anti-rabbit IgG (H+L) (DyLight™ 680 Conjugate)
Antibody Type Secondary Antibodies
Product description

Anti-rabbit IgG (H+L) was conjugated to DyLight™ 680 fluorescent dye under optimal conditions and formulated at 1 mg/ml. Excitation is 684 nm and peak fluorescence emission is 715 nm.

Key Feature

Clonality Monoclonal
Tested Applications

WB

Species Reactivity

Rabbit

Concentration 1 mg/ml
Purification

Target Information

Tissue Specificity

Anti-rabbit IgG (H+L) (DyLight™ 680 Conjugate) reacts with heavy and light chain of most rabbit immunoglobulins. No cross-reactivity to other serum proteins has been detected. This antibody may cross-react with immunoglobulins from other species.

Application

  • Application

    Western blot analysis of Jurkat cell lysates (#9194) treated with either U0126 (MEK 1/2 inhibitor) or TPA (12-O-Tetradecanoylphorbol-13-Acetate) using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb detected with Anti-rabbit IgG (H+L) (DyLight™ 680 Conjugate) (red) and Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb detected with Anti-mouse IgG (H+L) (DyLight™ 800 Conjugate) (green). The array image pixel intensities obtained using a LI-COR ® Biosciences Odyssey ® Infrared Imaging System are shown in the upper panel while corresponding fluorescent western blots are shown in the lower panel.

  • Application

    In-Cell Western™ analysis of A549 cells exposed to varying concentrations of U0126 (MEK1/2 Inhibitor) for 3 hours, followed by TPA (Phorbol-12-Myristate-13-Acetate) stimulation for 30 minutes. With increasing concentrations of U0126, a significant decrease (~5 fold) in Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP ® Rabbit mAb signal as compared to the TPA-stimulated control was observed. When using phospho-Erk as a measurement, the IC 50 of this compound was 1.9 μM. Data and images were generated on the LI-COR ® Biosciences Odyssey ® Infrared Imaging System using Anti-rabbit IgG (H+L) (DyLight™ 680 Conjugate).

Additional Information

Storage Instructions

The optimal dilution of the anti-species antibody should be determined by the user. However, the final dilutions below should yield acceptable results for the respective applications.

Fluorescent western blotting: 1:15000

In-Cell Western: 1:500

Storage: Supplied in 100 mM PBS, pH 7.2, containing 1% BSA and 0.02% sodium azide. Store at 4°C. Protect from light. Do not freeze.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

  • Catalog Number: OM296285
  • Price: $150.00
  • Discount Price
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  • Store: Out of stock
  • Lead time: 4~6 Week
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