All products Antibodies Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate)

Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate)

Catalog Number：OM296280

  • Confocal immunofluorescent analysis of P19 cells using LIN28A Antibody detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).

  • Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002, Wortmannin and U0126 (blue), using Phospho-Akt (Ser473) (D9E) Rabbit mAb detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) and compared to a nonspecific negative control antibody (red).

Product Profile

Product Name Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate)
Antibody Type Secondary Antibodies
Product description 
Anti-Rabbit IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and formulated at 1 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.

Key Feature

Clonality Monoclonal
Isotype Cocktail
Host Species Goat
Tested Applications

ChIPFCIFIHCIPWB
The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays.:
Species Reactivity

Rabbit
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Tissue Specificity F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins and purified human paraproteins.

Application

  • Application

    Confocal immunofluorescent analysis of P19 cells using LIN28A Antibody detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).

  • Application

    Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002, Wortmannin and U0126 (blue), using Phospho-Akt (Ser473) (D9E) Rabbit mAb detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) and compared to a nonspecific negative control antibody (red).

Application Notes
The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays.:

Additional Information

Form Liquid
Storage Instructions Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM296280
  • Price: $150.00
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  • Store: Out of stock
  • Lead time: 4~6 Week

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