Confocal immunofluorescent analysis of P19 cells using LIN28A Antibody detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002, Wortmannin and U0126 (blue), using Phospho-Akt (Ser473) (D9E) Rabbit mAb detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) and compared to a nonspecific negative control antibody (red).
Anti-Rabbit IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and formulated at 1 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.
Key Feature
Clonality
Monoclonal
Isotype
Cocktail
Host Species
Goat
Tested Applications
ChIPFCIFIHCIPWB
The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays.:
Species Reactivity
Rabbit
Concentration
1 mg/ml
Purification
Affinity purified
Target Information
Tissue Specificity
F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins and purified human paraproteins.
Application
Application
Confocal immunofluorescent analysis of P19 cells using LIN28A Antibody detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).
Application
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002, Wortmannin and U0126 (blue), using Phospho-Akt (Ser473) (D9E) Rabbit mAb detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) and compared to a nonspecific negative control antibody (red).
Application Notes
The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays.:
Additional Information
Form
Liquid
Storage Instructions
Storage:
Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
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