All products Antibodies Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate)

Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate)

Catalog Number:OM296281

  • Confocal immunofluorescent analysis of HeLa cells labeled with MEK1/2 (47E6) Rabbit mAb detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate) (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin (green). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).

  • Confocal immunofluorescent analysis of mouse cerebellum using α-Synuclein Antibody (IF Preferred) detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate) (red) and Neurofilament-L (DA2) Mouse mAb detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) (green). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).

Product Profile

Product Name Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate)
Antibody Type Secondary Antibodies
Product description

Anti-Rabbit IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 555 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.

Key Feature

Clonality Monoclonal
Tested Applications

WBIPIHCIFFCChIP

Species Reactivity

Rabbit

Concentration 1 mg/ml
Purification

Target Information

Tissue Specificity

F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins and purified human paraproteins.

Application

  • Application

    Confocal immunofluorescent analysis of HeLa cells labeled with MEK1/2 (47E6) Rabbit mAb detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate) (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin (green). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).

  • Application

    Confocal immunofluorescent analysis of mouse cerebellum using α-Synuclein Antibody (IF Preferred) detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 555 Conjugate) (red) and Neurofilament-L (DA2) Mouse mAb detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) (green). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).

Additional Information

Storage Instructions

The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:1000 should yield acceptable results for immunofluorescent assays.

Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

  • Catalog Number: OM296281
  • Price: $150.00
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  • Store: Out of stock
  • Lead time: 4~6 Week
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