All products Antibodies Anti-rat IgG (H+L), (Alexa Fluor ® 488 Conjugate)

Anti-rat IgG (H+L), (Alexa Fluor ® 488 Conjugate)

Catalog Number:OM296305

  • Confocal immunofluorescent analysis of HeLa cells, untreated (left) or heat shocked (right), using HSP70 (6B3) Rat mAb detected with Anti-rat IgG (H+L), (Alexa Fluor ® 488 Conjugate) (green). Actin filaments have been labeled with Alexa Fluor ® 555 phalloidin (red).

  • Flow cytometric analysis of HeLa cells using HSP70 (6B3) Rat mAb detected with Anti-rat IgG (H+L), (Alexa Fluor ® 488 Conjugate) (green) and compared to a nonspecific negative control antibody (red).

Product Profile

Product Name Anti-rat IgG (H+L), (Alexa Fluor ® 488 Conjugate)
Antibody Type Secondary Antibodies
Product description
Anti-rat IgG (H+L) antibody was conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and formulated at 2 mg/ml.

Key Feature

Clonality Monoclonal
Isotype Cocktail
Host Species Alligator
Tested Applications

ChIPFCIFIHCIPWB

Species Reactivity

Rat

Concentration 1 mg/ml
Purification Affinity purified

Target Information

Tissue Specificity IgG is prepared from goat antibodies that have been adsorbed against mouse IgG, mouse serum and human serum.

Application

  • Application

    Confocal immunofluorescent analysis of HeLa cells, untreated (left) or heat shocked (right), using HSP70 (6B3) Rat mAb detected with Anti-rat IgG (H+L), (Alexa Fluor ® 488 Conjugate) (green). Actin filaments have been labeled with Alexa Fluor ® 555 phalloidin (red).

  • Application

    Flow cytometric analysis of HeLa cells using HSP70 (6B3) Rat mAb detected with Anti-rat IgG (H+L), (Alexa Fluor ® 488 Conjugate) (green) and compared to a nonspecific negative control antibody (red).

Additional Information

Storage Instructions The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution shown below should yield acceptable results for immunofluorescent and flow cytometry assays:Immunofluorescent: 1:500 - 1:2000Flow Cytometry: 1:500 - 1:1000 Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

  • Catalog Number: OM296305
  • Price: $150.00
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