Confocal immunofluorescent analysis of HeLa cells using RPA32 (4E4) Rat mAb detected with Anti-rat IgG (H+L) (Alexa Fluor ® 555 Conjugate) (red) and COX IV (3E11) Rabbit mAb detected with Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) (green). Actin filaments have been labeled with Alexa Fluor ® 647 phalloidin (blue).
Anti-rat IgG (H+L) antibody was conjugated to Alexa Fluor® 555 fluorescent dye under optimal conditions and formulated at 2 mg/mL.
Key Feature
Clonality
Monoclonal
Isotype
Cocktail
Host Species
Alligator
Tested Applications
ChIPFCIFIHCIPWB
Species Reactivity
Rat
Concentration
1 mg/ml
Purification
Affinity purified
Target Information
Tissue Specificity
IgG is prepared from goat antibodies that have been adsorbed against mouse IgG, mouse serum and human serum.
Application
Application
Confocal immunofluorescent analysis of HeLa cells using RPA32 (4E4) Rat mAb detected with Anti-rat IgG (H+L) (Alexa Fluor ® 555 Conjugate) (red) and COX IV (3E11) Rabbit mAb detected with Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) (green). Actin filaments have been labeled with Alexa Fluor ® 647 phalloidin (blue).
Additional Information
Storage Instructions
The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent assays.
Storage:
Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
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