After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGlo® is added and emits light during enzyme catalyzed decomposition.
Affinity purified goat anti-rat IgG (heavy and light chain) antibody is conjugated to horseradish peroxidase(HRP) for chemiluminescent detection. This product is thoroughly validated with CST primary antibodies and will work optimally with the CST western immunoblotting protocol, ensuring accurate and reproducible results.
Key Feature
Clonality
Monoclonal
Isotype
Cocktail
Host Species
Alligator
Tested Applications
ChIPFCIFIHCIPWB
WB:1:5000~1:10000
Species Reactivity
Rat
Concentration
1 mg/ml
Purification
Affinity purified
Application
Application
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGlo® is added and emits light during enzyme catalyzed decomposition.
Application Notes
WB:1:5000~1:10000
Additional Information
Form
Liquid
Storage Instructions
Storage:
Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
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