All products Antibodies CBP Antibody (C-20)

CBP Antibody (C-20)

Catalog Number：OM157627

  • Western blot analysis of CBP expression in Jurkat nuclear extract.

  • Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovary tissue showing nuclear and cytoplasmic staining of cells in fallopian tubes.

  • Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with CBP (C-20), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .

  • Control Input (A). Antibodies used included RORα (C-16): (B), TIP60 (N-17): (C), CBP (A-22): and CBP (C-20): (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131.

  • Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , CBP (A-22): , CBP (C-1): , CBP (C-20): , CBP (451): , p300 (C-20): 585, p300 (N-15): 584, p300 (H-272): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.

  • Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , Bcl-3 (C-14): , Bcl-3 (H-146): , PCAF (C-16): , PCAF (H-369): , CBP (A-22): , CBP (C-1): , CBP (C-20): , CBP (451): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.

  • Antibodies tested include β-catenin (H-102): , β-catenin (C-18): , β-catenin (E-5): , Tip60 (N-17): , TRRAP (T-17): , TRRAP (Y-18): , TRRAP (F-20): , TRRAP (H-300): , CBP (A-22): , CBP (C-20): , CBP (451): , CPB (C-1): , p300 (H-272): , p300 (N-15): and p300 (C-20): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Kioussi et al., Cell 2002, 111: 673-685.

  • Immunofluorescence staining of methanol-fixed Jurkat cells showing nuclear staining.

  • Immunoperoxidase staining of formalin-fixed, paraffin-embedded human pancreas tissue showing nuclear staining.

Product Profile

Product Name CBP Antibody (C-20)
Antibody Type Primary Antibodies
Modification Notes Cyclic AMP-regulated gene expression frequently involves a DNA element designated the cAMP-regulated enhancer (CRE). Many transcription factors bind to this element, including the protein CREB, which is activated as a result of phosphorylation by protein kinase A. It has been shown that protein kinase A-mediated CREB phosphorylation results in its binding to a nuclear protein designated CBP (for CREB-binding protein). These findings suggest that CBP has many of the properties expected of a CREB co-activator. Another high molecular weight transcriptional adapter protein, designated p300, is characterized by three cysteine- and histidine-rich regions, of which the most carboxy terminal region specifically binds the adenovirus E1A protein. p300 molecules lacking an intact E1A binding site bypass E1A repression, even in the presence of high concentrations of E1A. Sequence analysis of CBP and p300 has revealed substantial homology, arguing that these proteins are members of a conserved family of co-activators.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBIFIHCFCELISAGSChIP
recommended for detection of CBP p265 of mouse, rat and human origin by WB, IF, IHC(P), ELISA and indirect FCM using PE-conjugated goat anti-rabbit IgG: sc-3739; also reactive with additional species, including equine, canine, bovine, porcine and avian:
Species Reactivity

HumanMouseRat
Concentration 1mg/ml
Purification Affinity purified

Target Information

Alternative Names
epitope mapping at the C-terminus of CBP of human origin
Tissue Specificity epitope mapping at the C-terminus of CBP of human origin

Database Links

Entrez Gene 1387

Application

  • Application

    Western blot analysis of CBP expression in Jurkat nuclear extract.

  • Application

    Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovary tissue showing nuclear and cytoplasmic staining of cells in fallopian tubes.

  • Application

    Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with CBP (C-20), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .

  • Application

    Control Input (A). Antibodies used included RORα (C-16): (B), TIP60 (N-17): (C), CBP (A-22): and CBP (C-20): (D). Data kindly provided by M.G. Rosenfeld and reproduced wtih permission from Gold et al., Neuron 2003, 40: 1119-1131.

  • Application

    Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , CBP (A-22): , CBP (C-1): , CBP (C-20): , CBP (451): , p300 (C-20): 585, p300 (N-15): 584, p300 (H-272): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.

  • Application

    Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , Bcl-3 (C-14): , Bcl-3 (H-146): , PCAF (C-16): , PCAF (H-369): , CBP (A-22): , CBP (C-1): , CBP (C-20): , CBP (451): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.

  • Application

    Antibodies tested include β-catenin (H-102): , β-catenin (C-18): , β-catenin (E-5): , Tip60 (N-17): , TRRAP (T-17): , TRRAP (Y-18): , TRRAP (F-20): , TRRAP (H-300): , CBP (A-22): , CBP (C-20): , CBP (451): , CPB (C-1): , p300 (H-272): , p300 (N-15): and p300 (C-20): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Kioussi et al., Cell 2002, 111: 673-685.

  • Application

    Immunofluorescence staining of methanol-fixed Jurkat cells showing nuclear staining.

  • Application

    Immunoperoxidase staining of formalin-fixed, paraffin-embedded human pancreas tissue showing nuclear staining.

Application Notes
recommended for detection of CBP p265 of mouse, rat and human origin by WB, IF, IHC(P), ELISA and indirect FCM using PE-conjugated goat anti-rabbit IgG: sc-3739; also reactive with additional species, including equine, canine, bovine, porcine and avian:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM157627
  • Price: $336.00
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