All products Antibodies ERK 3 Antibody (I-15) PE

ERK 3 Antibody (I-15) PE

Catalog Number:OM154755

  • Intracellular FCM analysis of fixed and permeabilized A-431 cells. Black line histogram represents the isotype control, normal rabbit IgG: .

  • Western blot analysis of ERK 3 expression in non-transfected: (A) and human ERK 3 transfected: (B) 293T whole cell lysates.

  • Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

  • Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

  • Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .

  • Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .

  • Western blot analysis of ERK 3 expression in PC-12 whole cell lysate.

  • Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tumor.

Product Profile

Product Name ERK 3 Antibody (I-15) PE
Antibody Type Primary Antibodies
Modification Notes Mitogen-activated protein kinase (MAPK) signaling pathways involve closely related MAP kinases, including extracellular-signal-related kinase 3 (ERK 3, also designated PRKM6 and p97MAPK). Serum, growth factors and phorbol esters can initiate ERK 3 signaling pathways. Despite lacking a definitive nuclear localization sequence, ERK 3 constitutively localizes to the nucleus upon activation. p38 pathway activation-dependent upregulation of ERK 3 is independent of the status of p53, Bcl-2 and caspase-3 during cell stress and damage induced by proteasome inhibition, suggesting ERK 3 in part mediates intracellular defense or cell rescue. The human ERK 3 gene maps to chromosome 15q21.2 and encodes a 721 amino acid protein.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

ELISAFCIFIHCIPWB

WB:1:100~1:500
IP:1:20~1:100
IF:1:20~1:100
IHC:1:10~1:50
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Alternative Names
epitope mapping within an internal region of ERK 3 of rat origin
Tissue Specificity epitope mapping within an internal region of ERK 3 of rat origin

Database Links

Entrez Gene 5597

Application

  • Application

    Intracellular FCM analysis of fixed and permeabilized A-431 cells. Black line histogram represents the isotype control, normal rabbit IgG: .

  • Application

    Western blot analysis of ERK 3 expression in non-transfected: (A) and human ERK 3 transfected: (B) 293T whole cell lysates.

  • Application

    Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

  • Application

    Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

  • Application

    Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .

  • Application

    Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .

  • Application

    Western blot analysis of ERK 3 expression in PC-12 whole cell lysate.

  • Application

    Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tumor.

Application Notes
WB:1:100~1:500
IP:1:20~1:100
IF:1:20~1:100
IHC:1:10~1:50

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

  • Catalog Number: OM154755
  • Price: $336.00
  • Discount Price
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  • Store: Out of stock
  • Lead time: 4~6 Week
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