GFP Tag Antibody

Western blot analysis of anti-GFP Mab (Cat. #AM1009a) using purified GFP, YFP and BFP proteins expressed in bacteria: Both GFP (Lanes 1-3) and YFP (Lanes 4-6) but not BFP (data not shown) were detected using the purified Mab.

Western blot analysis of anti-GFP Tag Antibody (Cat. #AM1009a) in GFP recombinant protein. GFP recombinant protein (arrow) was detected using the purified Mab.

Immuno-fluorescence for GFP Tag Antibody (Cat # AM1009A). Antibody target is a YFP tagged protein (the inner membrane complex) of Toxoplasma gondii, which localizes around the exterior of the parasite. Brighter parasites represent actively dividing cells and darker ones are not actively dividing.Secondary goat-anti-mouse and goat-anti-rabbit were conjugated with Alexa546 (Molecular Probes)at 1:200 dilution. Phase contrast enhancement applied. Fluorescent lamp exposure times as follows: RFP Channel, 0.075 sec; YFP Channel, 1.000 sec; DAPI Channel, 0.020 sec; Phase Channel: 0.300 sec. Microscope: Axiovert 200M inverted microscope (Zeiss) equipped with a PRIOR stage and a NEOFLUAR 100x/1.3 objective lens. Images were collected with a Hamamatsu C4742-95 CCD camera. Chroma Filtersets: TRITC, HQ545/30x – Q570LP – HQ620/60m; DAPI, D350/50x – E420LP – 400DCLP; YFP, Q151LP – HQ500/20x – HQ535/30m. Data courtesy of Seth Robertson, Boston College.

Formalin-fixed and paraffin-embedded mouse skin tissue expression GFP reacted with GFP Tag Antibody (Cat.#AM1009a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. -ve shows negative staining of the antibody in a wild type mouse, whereas +ve shows positive staining in a mouse expressing GFP under the LGR5 promoter. The staining shows localization of GFP at the bottom of the hair follicle. The antigen was retrieved in citrate pH 6 in water bath for 25 mins.